Difference between revisions of "Part:BBa K627012"

Revision as of 16:31, 21 September 2011

Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

This construct contains the cleavage site for the TEV protease flanked by the sequence of beta-lactamase and the TorA signal sequence for the TAT (twin arginine transporter) pathway, thus in case of the expression of the complete construct the beta lactamase can be transported into periplasm and provide ampicillin resistance. The TorA signal-sequence originate from the enzyme Trimethylamin-N-Oxid-Reduktase , which needs to be folded in the cytoplasm. The beta lactamase is an enzyme which cleaves lactam rings and makes bacteria resistant to antibiotics like ampicillin and penicillin. The cleavage site for the TEV protease was created via oligo hybridization and ligated into the construct with cleavage site for XhoI and NheI, which makes this part modular and easy adaptable for any other protease.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 117
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 143
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 796

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 __NOTOC__
 <partinfo>BBa_K627012 short</partinfo>
+This construct contains the cleavage site for the TEV protease flanked by the sequence of beta-lactamase and the TorA signal sequence for the TAT (twin arginine transporter) pathway, thus in case of the expression of the complete construct the beta lactamase can be transported into periplasm and provide ampicillin resistance. The TorA signal-sequence originate from the enzyme Trimethylamin-N-Oxid-Reduktase , which needs to be folded in the cytoplasm. The beta lactamase is an enzyme which cleaves lactam rings and makes bacteria resistant to antibiotics like ampicillin and penicillin. The cleavage site for the TEV protease was created via oligo hybridization and ligated into the construct with cleavage site for XhoI and NheI, which makes this part modular and easy adaptable for any other protease.