Difference between revisions of "Part:BBa K627007"

Revision as of 19:33, 21 September 2011

Fusion of c-myc-tag and gene III

The gene III protein is a coat protein from the filamentous bacteriophage M13. It appears only 3-5 times on the tip of the phage and is responsible for infection of bacterial cells. It is usually fused to the gene of interest to determine protein-protein-interactions of its expression product using M13 phage display system. The gene III-protein consists of three domains: one C-termial and two N-terminal domains ( Holliger and Riechmann, 1997). Gene III was generated as biobrick several times for instance M13003 (2006) and M31537 (2007). It has been shown that proteins of interest fused to the carboxy-terminus of gene-III-proteins can be functionally displayed on the surface of phages (Fuh, 2000 and Rakonjak, 1999). In the course of our project a phagemid was constructed carrying truncated geneIII (only C-terminus) as biobrick. To enable easy purification and detection of gene-III-fused proteins additionally a myc-tag-sequence was merged before gene –III-sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K627007 short</partinfo>
+The gene III protein is a coat protein from the filamentous bacteriophage M13. It appears only 3-5 times on the tip of the phage and is responsible for infection of bacterial cells. It is usually fused to the gene of interest to determine protein-protein-interactions of its expression product using M13 phage display system. The gene III-protein consists of three domains: one C-termial and two N-terminal domains ( Holliger and Riechmann, 1997).
+Gene III was generated as biobrick several times for instance M13003 (2006) and M31537 (2007). It has been shown that proteins of interest fused to the carboxy-terminus of gene-III-proteins can be functionally displayed on the surface of phages (Fuh, 2000 and  Rakonjak, 1999). In the course of our project a phagemid was constructed carrying truncated geneIII (only C-terminus) as biobrick. To enable easy purification and detection of gene-III-fused proteins additionally a myc-tag-sequence was merged before gene –III-sequence.