Difference between revisions of "Part:BBa K676013:Design"
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Revision as of 21:55, 21 September 2011
Mu/Spy Ligation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1203
Design Notes
AFter the PCR and ligation of Mu GBS into a pSB1C3 backbone, we carried out 3A Assembly of the Mu GBS with the SPY device in BBa_K239009 into a pSB1A3
Source
2009 UCL SPY device