Difference between revisions of "Part:BBa K676001:Experience"
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Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better . The uniformity or homogeneity of the supercoiled target plasmids increases by close to 40% compared to the control. | Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better . The uniformity or homogeneity of the supercoiled target plasmids increases by close to 40% compared to the control. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:14, 21 September 2011
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Applications of BBa_K676001
According to our result in the 1D chloroquine gel electrophoresis ( at 2.5 microgram /mL) , the plasmid with the Mu phage GBS( pSB1C3 backbone) migrated further compared to the control plasmid ( pSB1C3 with RFP expression cassette) . At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better . The uniformity or homogeneity of the supercoiled target plasmids increases by close to 40% compared to the control.
User Reviews
UNIQ7faa9fb4f3e3d537-partinfo-00000000-QINU UNIQ7faa9fb4f3e3d537-partinfo-00000001-QINU