Difference between revisions of "Part:BBa K676013"
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The Mu GBS which was ligated into the BBa_K239009 SPY device shows a higher expression of GFP in vivo compared to the SPY device without the Mu GBS . The data was plotted into a graph and presented as a characterisation of device in our project. The higher expression level may due to the fact that negative supercoiling in the DNA promote easier transition from the close promoter complex to open promoter complex for higher rate of transcription. The higher level of expression means that the introduction of such GBS into DNA vaccine or gene therapy plasmid will be able to trigger a better immune response for a fully developed protection against the target infection .Although this is only in theory , further studies will be required to vertify it. | The Mu GBS which was ligated into the BBa_K239009 SPY device shows a higher expression of GFP in vivo compared to the SPY device without the Mu GBS . The data was plotted into a graph and presented as a characterisation of device in our project. The higher expression level may due to the fact that negative supercoiling in the DNA promote easier transition from the close promoter complex to open promoter complex for higher rate of transcription. The higher level of expression means that the introduction of such GBS into DNA vaccine or gene therapy plasmid will be able to trigger a better immune response for a fully developed protection against the target infection .Although this is only in theory , further studies will be required to vertify it. | ||
| − | The higher level of expression can be use in making a more sensitive SPY device in order to detect shear stress during fermentation as well as downstream processing. | + | The higher level of expression can be use in making a more sensitive SPY device in order to detect shear stress during fermentation as well as in downstream processing. |
| − | As the BBa_K676001( Mu GBS ) , we also observed a better uniformity in the supercoiled plasmids ( Mu GBS with SPY device) . Although the extent of supercoiling in the plasmid with and without the GBS only showed a little difference but the GBS provided us with a more uniformly supercoiled plasmid products. | + | As with the BBa_K676001( Mu GBS ) , we also observed a better uniformity in the supercoiled plasmids ( Mu GBS with SPY device) . Although the extent of supercoiling in the plasmid with and without the GBS only showed a little difference but the GBS provided us with a more uniformly supercoiled plasmid products. |
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Revision as of 12:07, 21 September 2011
Mu/Spy Ligation
A device to detects the level of stress due to a GBS being present on the plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1203