Difference between revisions of "Part:BBa K564012"
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ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The 5' untranslated region and promoter of this gene is fused with reporter gene lacZ and is donwlregulated by sRNA chiX (also known as micM or sroB).
 
ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The 5' untranslated region and promoter of this gene is fused with reporter gene lacZ and is donwlregulated by sRNA chiX (also known as micM or sroB).
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 +
{| class="wikitable"
 +
|-
 +
! Strain
 +
! [http://ecoliwiki.net/colipedia/index.php/NM522 NM522]
 +
! [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
 +
|-
 +
| [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564012 '''PchiP(original):lacZ, BBa_K564012''']
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| 467
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| 4781
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|-
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| [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564013 '''PchiP(mutated):lacZ, BBa_K564013''']
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| 2353
 +
| 2403
 +
|}
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:34, 21 September 2011

promoter and RBS of E. coli chitoporin translationally fused with lacZ.

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The 5' untranslated region and promoter of this gene is fused with reporter gene lacZ and is donwlregulated by sRNA chiX (also known as micM or sroB).

Strain [http://ecoliwiki.net/colipedia/index.php/NM522 NM522] [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
PchiP(original):lacZ, BBa_K564012 467 4781
PchiP(mutated):lacZ, BBa_K564013 2353 2403

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]