Difference between revisions of "Part:BBa K592200"

Revision as of 11:29, 21 September 2011

Low to medium copy BioBrick standard vector

BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3116
Illegal NheI site found at 1403
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3122
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3116
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3116
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3116
Plasmid lacks a suffix.
Illegal XbaI site found at 3131
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1969
Illegal AgeI site found at 994
Illegal AgeI site found at 1317
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 289

Revision as of 11:28, 21 September 2011 (view source) Antonio (Talk \| contribs) ← Older edit		Revision as of 11:29, 21 September 2011 (view source) Antonio (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K592200 short</partinfo>		<partinfo>BBa_K592200 short</partinfo>

−	BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The ''E coli'' His operon terminator BBa_B0053 has been replaced with the late terminator of the ''Salmonella'' phage P22.	+	BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in ''E coli''. The ''E coli'' His operon terminator BBa_B0053 has been replaced with the late terminator of the ''Salmonella'' phage P22.