Difference between revisions of "Part:BBa K592001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. | + | This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. The removed sites are: A1282C and A1284C to remove EcoRI site, and T1383C to remove SpeI site. Furthermore, while removing the EcoRI site, G1278T was also done to exchange a rare codon (GGG to GGT). |
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===Source=== | ===Source=== | ||
Revision as of 09:04, 21 September 2011
CcaS, green light sensor from Synechocystis sp. PCC6803
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 606
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2252
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1270
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. The removed sites are: A1282C and A1284C to remove EcoRI site, and T1383C to remove SpeI site. Furthermore, while removing the EcoRI site, G1278T was also done to exchange a rare codon (GGG to GGT).
Source
Synechocystic sp. PCC6803