Difference between revisions of "Part:BBa K590017"
(→‎Usage and Biology)
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[[Image:IGEM11 HIEJKL partregistry.png|500px|center]]
 
[[Image:IGEM11 HIEJKL partregistry.png|500px|center]]
  
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Cell chain formation was observed in microscopy when this plasmid was transformed in ''E.coli''.  
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Below is the gel image of HIEJKL(~9000bp), we extracted this band and "gibsoned" it with pGA3k3 plasmid backbone.
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[[Image:Igem2011 HIEJKL gel.png|thumb|center|1kb Ladder (left), mamHIEJKL (right)]]
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After Gibson cloning and transformation, cell chain formation was observed in microscopy when this plasmid was transformed in ''E.coli''.  
  
 
[[Image:HIEJKL 1b wiki.png|500px|center]]
 
[[Image:HIEJKL 1b wiki.png|500px|center]]

Revision as of 06:53, 22 September 2011

mamHIEJKL in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes mamHI, mamE, mamJ, mamKL in a pGA3k3 plasmid backbone. All the genes were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1 on a pGA3k3 plasmid backbone.

Usage and Biology

Made by Gibson Cloning method instead of Standard Biobrick method, this part is in low copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit], known as the "first part of the full assembly".

IGEM11 HIEJKL partregistry.png

Below is the gel image of HIEJKL(~9000bp), we extracted this band and "gibsoned" it with pGA3k3 plasmid backbone.

1kb Ladder (left), mamHIEJKL (right)

After Gibson cloning and transformation, cell chain formation was observed in microscopy when this plasmid was transformed in E.coli.

HIEJKL 1b wiki.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal BsaI.rc site found at 6947
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491
    Illegal SapI.rc site found at 9208
    Illegal SapI.rc site found at 9529