Difference between revisions of "Part:BBa K568005"
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Cloning intermediate. Last step of assembly. | Cloning intermediate. Last step of assembly. | ||
− | This is an intermediate cloning construct. For | + | This is an intermediate cloning construct. For (<html><a href="https://parts.igem.org/Part:BBa_K568001">BBa_K568001</a></html>), the final part, T7ptag, a T7 polymerase with amber stop codon mutation, (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html>) needs to be added. |
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 22:06, 20 September 2011
Intermediate optogenetical AND-Gate without T7ptag
Cloning intermediate. Last step of assembly. This is an intermediate cloning construct. For (BBa_K568001), the final part, T7ptag, a T7 polymerase with amber stop codon mutation, (BBa_K228000) needs to be added.
Usage and Biology
see Part (BBa_K568001)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3820