Difference between revisions of "Part:BBa K568003"
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This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part.  
 
This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part.  
 
The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.
 
The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.
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===Experimental Testing===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:27, 21 September 2011

T7 promoter lacZ reporter part

beta-galactosidase expression upon T7 polymerase binding


Usage and Biology

This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part. The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.


Experimental Testing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]