Difference between revisions of "Part:BBa K525224:Design"

(→‎Design Notes)
(→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
Corynebacterium halotolerans
+
* PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
 +
** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
 +
*** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGACGCCCAGGTTGCCAC
 +
*** rev: CTGCAGCGGCCGCTACTAGTATTAGATGCCCAGGCCACCGACG
  
 
===References===
 
===References===

Revision as of 20:44, 20 September 2011

S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 440
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 440
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1191
    Illegal XhoI site found at 647
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 440
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 440
    Illegal NgoMIV site found at 314
    Illegal NgoMIV site found at 1403
    Illegal AgeI site found at 89
    Illegal AgeI site found at 305
    Illegal AgeI site found at 546
    Illegal AgeI site found at 593
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 995
    Illegal BsaI.rc site found at 302
    Illegal BsaI.rc site found at 680
    Illegal BsaI.rc site found at 1082


Design Notes

This is the cspB S-layer sequence with TAT sequence and without lipid anchor.

Source

  • PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGACGCCCAGGTTGCCAC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGATGCCCAGGCCACCGACG

References