Difference between revisions of "Part:BBa K525121"
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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S-layer proteins can be used as scaffold for nanobiotechnological applications and devices by e.g. fusing the S-layer's self-assembly domain to other functional protein domains. It is possible to coat surfaces and liposomes with S-layers. A big advantage of S-layers: after expressing in ''E. coli'' and purification, the nanobiotechnological system is cell-free. This enhances the biological security of a device.
  
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This fluorescent S-layer fusion protein is used to characterize purification methods and the S-layer's ability to self-assemble on surfaces.
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===Important parameters===
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<center>
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{|{{Table}}
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!Experiment
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!Characteristic
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!Result
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|-
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|rowspan="3"|[[Part:BBa_K525305#Expression_in_E._coli | Expression (''E. coli'')]]
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|Localisation
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|cell membrane
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|-
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|Compatibility
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|''E. coli'' KRX
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|-
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|Inductor for expression
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|L-rhamnose for induction of T7 polymerase
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|-
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|rowspan="3"|[[Part:BBa_K525305#Purification_of_SgsE_fusion_protein | Purification]]
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|Molecular weight
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|
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|-
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|Theoretical pI
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|
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|-
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|}
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</center>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:13, 21 September 2011

S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1421
    Illegal XhoI site found at 248
    Illegal XhoI site found at 866
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400
    Illegal SapI site found at 647
    Illegal SapI site found at 859
    Illegal SapI site found at 1407