Difference between revisions of "Part:BBa K525121:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This is the complete cspB S-Layer sequence with TAT sequence and lipid anchor.
+
This is the complete cspB S-Llyer sequence with TAT sequence and lipid anchor.
  
 
===Source===
 
===Source===

Revision as of 20:06, 20 September 2011

S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1421
    Illegal XhoI site found at 248
    Illegal XhoI site found at 866
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400
    Illegal SapI site found at 647
    Illegal SapI site found at 859
    Illegal SapI site found at 1407


Design Notes

This is the complete cspB S-Llyer sequence with TAT sequence and lipid anchor.

Source

  • PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGTTTAACAACCGTATCCGCAC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
  • [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]

References

Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J (2004) Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy [http://www.sciencedirect.com/science/article/pii/S016816560400241X J Biotechnol. 26;112(1-2):177-93.]