Difference between revisions of "Part:BBa K540000:Experience"
| Line 24: | Line 24: | ||
<div style="text-align:center; float:left; margin-left:30px; margin-top:-20px;"> | <div style="text-align:center; float:left; margin-left:30px; margin-top:-20px;"> | ||
| − | <img src="http://i.imgur.com/wnOY5.png" style="width:525px; height:350px; margin-top: | + | <img src="http://i.imgur.com/wnOY5.png" style="width:525px; height:350px; margin-top: 100px; margin-bottom: 5px; position: relative;"/> |
</div> | </div> | ||
| Line 37: | Line 37: | ||
<div style="text-align:center; float:right; margin-left:30px; margin-top:-20px;"> | <div style="text-align:center; float:right; margin-left:30px; margin-top:-20px;"> | ||
| − | <img src="http://i.imgur.com/zIwBU.png" style="width:481px; height:418px; margin-top: | + | <img src="http://i.imgur.com/zIwBU.png" style="width:481px; height:418px; margin-top: 60px; margin-bottom: 5px; position: relative;"/> |
</div> | </div> | ||
Revision as of 19:07, 20 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K540000
A preliminary search on the best condition for use was carried out at first.
Antibiotic response
Two 24 wells plates have been seeded with the same strains, in LB/2 medium. Ampicilline for S18 and Cm have been added only in the second plate.
The results have examined after 24 hours of incubation 30°C.
Qualitative approach
As can be seen on the right, adding ampicilline boosts the adherence of the strain, and makes it more cobalt responsive.
Quantitative approach
Furthermore, the adherence level was determined quantitatively using OD measurements. Resulting graphs are shown on the left.
P1 indicates the first plate, without antibiotics, while P2 indicates the second plate, with antibiotics.
It can clearly be seen that while the growth of the bacteria are similar, as they have similar total OD, adding the antibiotics makes them a lot more adherent, and enable the cobalt-response.
This can be explained by the fact that without antibiotics, the bacteria will tend to lose the part, and thus become unresponsive to cobalt.
===User Reviews===