Difference between revisions of "Part:BBa K620003"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K620003 short</partinfo> | <partinfo>BBa_K620003 short</partinfo> | ||
− | Allows for inducible expression of the protein BisdB, one of a pair of proteins that can degrade bisphenol A (BPA). | + | Allows for inducible expression of the protein BisdB, one of a pair of proteins that can degrade bisphenol A (BPA). It is controlled by the tet promoter which can be induced with tetracycline. It should be used in conjunction with [[Part:BBa_K620002|K620002]] to degrade BPA. |
+ | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | [[Image:Pnt002map.png|frame|center|Plasmid map of K620003 in pSB3K3]] | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 08:21, 28 September 2011
Tet promoter + BisdB
Allows for inducible expression of the protein BisdB, one of a pair of proteins that can degrade bisphenol A (BPA). It is controlled by the tet promoter which can be induced with tetracycline. It should be used in conjunction with K620002 to degrade BPA.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 545
Illegal BamHI site found at 933 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 70
Illegal AgeI site found at 1342 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1152