Difference between revisions of "Part:BBa K678062"

Revision as of 13:28, 20 September 2011

pJEJAM14

pJEJAM14 is a plasmid that after digestion with NotI is intended to be transformed into Aspergillus nidulans and integrated into the genome by non-homologous end joining. The plasmid consists the strong constitutive gpdA promoter , monomeric red fluorescent protein (mRFP1), the trpC terminator, a pyrG marker cassette for selection, an ori for propagation in E. coli, and an ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.

consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans with device DIC light.

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans with device DIC light.

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Front

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Back

Sequence and Features