Difference between revisions of "Part:BBa K515104:Experience"

(Applications of BBa_K515104)
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===Applications of BBa_K515104===
 
===Applications of BBa_K515104===
  
*The intended application of this construct was to negate the expression of holin within our <html><a href="http://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview"/>Gene Guard</a> system.
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*The intended application of this construct was to negate the expression of holin within our <html><a href="http://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview"/>Gene Guard</a></html> system.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 10:15, 20 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K515104

  • The intended application of this construct was to negate the expression of holin within our Gene Guard system.

User Reviews

UNIQ258e43e53f9519c7-partinfo-00000001-QINU UNIQ258e43e53f9519c7-partinfo-00000002-QINU

This part has been sequence verified. It has also been tested for protein expression. A nice clear band of the appropriate size can be seen at the bottom of the gel indicating a strong overexpression of a protein if this sequence is in a pSB1C3 plasmid.