Difference between revisions of "Part:BBa K546005:Experience"

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This part was tested by measuring overnight <I>E. coli</I> cultures transformed with high copy plasmid pSB1A2 containing this insert. Fluorescence was measured in a Molecular Devices Spectramax M2 spectrophotometer, exciting the samples at 485 nm and detecting at 510 nm. 200 µL samples were analyzed in an opaque 96-wells plate. The GFP expression was monitored for 2 hours, with BBa_K546001 transformants (high copy, pSB1A2) as negative control.  
 
This part was tested by measuring overnight <I>E. coli</I> cultures transformed with high copy plasmid pSB1A2 containing this insert. Fluorescence was measured in a Molecular Devices Spectramax M2 spectrophotometer, exciting the samples at 485 nm and detecting at 510 nm. 200 µL samples were analyzed in an opaque 96-wells plate. The GFP expression was monitored for 2 hours, with BBa_K546001 transformants (high copy, pSB1A2) as negative control.  
  
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The outcome is shown below:
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The outcome is shown below. R0063-F2621 refers to the repaired version of F2621, submitted as [https://parts.igem.org/Part:BBa_K546003 K546003].
  
 
[[Image: Fluorescence measurement BBa K546005-BBa K546001.png]]
 
[[Image: Fluorescence measurement BBa K546005-BBa K546001.png]]

Revision as of 20:51, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K546005

This part was used as the positive feedback loop for the synchronized oscillator based on “A synchronized quorum of genetic clocks” by Danino et al., 2010.

User Reviews

UNIQ151c51846be02d02-partinfo-00000000-QINU

No review score entered. Youri van Nuland

This part was tested by measuring overnight E. coli cultures transformed with high copy plasmid pSB1A2 containing this insert. Fluorescence was measured in a Molecular Devices Spectramax M2 spectrophotometer, exciting the samples at 485 nm and detecting at 510 nm. 200 µL samples were analyzed in an opaque 96-wells plate. The GFP expression was monitored for 2 hours, with BBa_K546001 transformants (high copy, pSB1A2) as negative control.

The outcome is shown below. R0063-F2621 refers to the repaired version of F2621, submitted as K546003.

Fluorescence measurement BBa K546005-BBa K546001.png

GFP expression rapidly increases over time, indicating a functional positive feedback loop.

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UNIQ151c51846be02d02-partinfo-00000002-QINU