Difference between revisions of "Part:BBa K510022"

Revision as of 23:07, 21 September 2011

attTn7

TnsABC+D transposition machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. Our [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Bioinformatics/attTn7_Insertion_Site Bioinformatic analysis] reveal that the insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] in any organism as well as in plasmids. All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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−	TnsABC+D ~~transposase~~ machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. ~~This~~ insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] in any organism as well as in plasmids.	+	TnsABC+D transposition machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. Our [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Bioinformatics/attTn7_Insertion_Site Bioinformatic analysis] reveal that the insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] in any organism as well as in plasmids.
	All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.		All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.