Difference between revisions of "Part:BBa K510018"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K510018 short</partinfo> | <partinfo>BBa_K510018 short</partinfo> | ||
− | The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a lacZ-alfa+GFP-AAV cassette (BBa_I732094) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism. | + | |
+ | The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a lacZ-alfa+GFP-AAV cassette ([https://parts.igem.org/Part:BBa_I732094 BBa_I732094]) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism. | ||
+ | |||
+ | [[Image:Mini-Tn7-Gm-I732094.png|700px|center]] | ||
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Revision as of 21:43, 19 September 2011
pUC18R6KT-miniTn7BB-Gm-lacZ+GFP
The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a lacZ-alfa+GFP-AAV cassette (BBa_I732094) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4528
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
Illegal BglII site found at 4560
Illegal BamHI site found at 4569 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal BsaI.rc site found at 5506
Illegal SapI site found at 518