Difference between revisions of "Part:BBa K510016"
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− | The mini-Tn7-Gm-pBad/araC artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/araC promoter(BBa_I0500) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism. | + | The mini-Tn7-Gm-pBad/araC artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a pBad/araC promoter([https://parts.igem.org/Part:BBa_I0500 BBa_I0500]) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism. |
[[Image:Mini-Tn7-Gm-I0500.png|700px|center]] | [[Image:Mini-Tn7-Gm-I0500.png|700px|center]] |
Revision as of 21:33, 19 September 2011
pUC18R6KT-miniTn7BB-Gm-pBad/araC
The mini-Tn7-Gm-pBad/araC artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/araC promoter(BBa_I0500) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4528
Illegal NheI site found at 5762
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
Illegal BamHI site found at 5701 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 5536 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518
Illegal SapI site found at 5518