Difference between revisions of "Part:BBa K510015"
Line 3: Line 3:
  
  
−
The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on '''pUC18R6KT-miniTn7-Gm''' (<a href="https://parts.igem.org/Part:BBa_K510012" title="BBa_K510012">BBa_K510012</a>) by inserting a RBS+RFP cassette (<a href="https://parts.igem.org/Part:BBa_K093005" title="BBa_K093005">BBa_K093005</a>) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism using the transposase machinery of the Tn7 transposon.
+
The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on '''pUC18R6KT-miniTn7-Gm''' ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a RBS+RFP cassette ([https://parts.igem.org/Part:BBa_K093005 BBa_K093005]) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism using the transposase machinery of the Tn7 transposon.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:58, 19 September 2011

pUC18R6KT-miniTn7BB-Gm-RBS+RFP


The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a RBS+RFP cassette (BBa_K093005) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism using the transposase machinery of the Tn7 transposon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5130
    Illegal AgeI site found at 5242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal SapI site found at 518