Difference between revisions of "Part:BBa K546002:Experience"

Revision as of 18:17, 19 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K546002

This part was used as an 3-oxohexanoyl-homoserine lactone (AHL) responsive element. lux pL yields low constitutive expression which results in luxR production. Complexed with luxR, AHL binds to the luxpR promoter which drives the expression of GFP.

User Reviews

UNIQ0fef3d46ea0c1be0-partinfo-00000000-QINU

No review score entered. Username

Functionality of this part was tested with a Spectramax M2 spectrophotometer. This apparatus can measure fluorescence in microplates. The response to AHL was examined by applying different concentrations of AHL: 0, 250 and 1000 nM AHL in a 200 µL of overnight culture samples susepended in PBS in a 96 wells plate. As a negative control for this experiment, part BBa_K546001 was used. This part should also respond to AHL, but without yielding a fluorescent signal.

;

UNIQ0fef3d46ea0c1be0-partinfo-00000002-QINU

@@ Line 9: / Line 9: @@
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K546002 StartReviews</partinfo>
-<!-- Template for a user review
+<!-- Template for a user review -->
 {|width='80%' style='border:1px solid gray'
 |-
@@ Line 16: / Line 16: @@
 <I>Username</I>
 |width='60%' valign='top'|
 Functionality of this part was tested with a Spectramax M2 spectrophotometer. This apparatus can measure fluorescence in microplates.
 The response to AHL was examined by applying different concentrations of AHL: 0, 250 and 1000 nM AHL in a 200 µL of overnight culture samples susepended in PBS in a 96 wells plate.
@@ Line 22: / Line 23: @@
 |};
-[[File:https://parts.igem.org/Image:AHL_response_test_BBa_K546002.png]]
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K546002 EndReviews</partinfo>