Difference between revisions of "Part:BBa K584024"
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<partinfo>BBa_K584024 short</partinfo>
 
<partinfo>BBa_K584024 short</partinfo>
  
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This part is the Ice Nucleating protien generator. It is composed of the ribosome binding site  [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 (RBS)], ice nucleating protein gene  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K584021 inaZ] and the two terminators [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010] [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012].  
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<p align="justify">This part is the Ice Nucleating protien generator. It is composed of the ribosome binding site  [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 (RBS)], ice nucleating protein gene  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K584021 inaZ] and the two terminators [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010] [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]. </p>
  
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The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.
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<p align="justify">The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.</p>
  
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We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.
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<p align="justify">We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.</p>
  
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To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.
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<p align="justify">To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.</p>
  
  

Revision as of 07:23, 21 September 2011

Ice Nucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

This part is the Ice Nucleating protien generator. It is composed of the ribosome binding site (RBS), ice nucleating protein gene inaZ and the two terminators BBa_B0010 BBa_B0012.

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 664
    Illegal NgoMIV site found at 2176
    Illegal NgoMIV site found at 2320
    Illegal NgoMIV site found at 2584
    Illegal NgoMIV site found at 2728
    Illegal NgoMIV site found at 2776
    Illegal NgoMIV site found at 2896
    Illegal AgeI site found at 435
    Illegal AgeI site found at 1840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 610
    Illegal BsaI.rc site found at 3356
    Illegal SapI.rc site found at 256