Difference between revisions of "Part:BBa K537012"

Revision as of 10:47, 19 September 2011

Promoter-Theophylline riboswitch 2-CheZ-Venus-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to CheZ and Venus fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The theophylline riboswitch2-CheZ fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-Venus fusion and CheZ-Venus fusion:Double terminator. The scar sequence is as follows: ACTAGA

A Freiburg fusion scar site exists between the CheZ and Venus sequence. The sequence is as follows: ACCGGC

Sequence and Features