Difference between revisions of "Part:BBa K649300"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K649300 short</partinfo> | <partinfo>BBa_K649300 short</partinfo> | ||
| − | This part has only rocF obtained from B.subtilis 168 by colony-PCR. | + | This part has only rocF obtained from <span style="font-style:italic;">B.subtilis</span> 168 by colony-PCR.<br /> |
| − | rocF is the cording region of Arginase, catalyzing the reaction from arginine to urea and ornithine. | + | rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.<br /> |
Original rocF has two EcoRI sites in ORF, so we changed these sites. | Original rocF has two EcoRI sites in ORF, so we changed these sites. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:44, 24 September 2011
Arginase (rocF)
This part has only rocF obtained from B.subtilis 168 by colony-PCR.
rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.
Original rocF has two EcoRI sites in ORF, so we changed these sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]