Difference between revisions of "Part:BBa K611010"
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<partinfo>BBa_K611010 short</partinfo> | <partinfo>BBa_K611010 short</partinfo> | ||
− | This part is used to characterize mutants of BBa_C0012 (LacI). By adding a mutant of BBa_C0012 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added | + | This part is used to characterize mutants of BBa_C0012 (LacI). By adding a mutant of BBa_C0012 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added LacI mutant can be varied through different arabinose concentrations as long as AraC is present. |
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Revision as of 00:05, 19 September 2011
LacI Repressor Characterization Construct
This part is used to characterize mutants of BBa_C0012 (LacI). By adding a mutant of BBa_C0012 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added LacI mutant can be varied through different arabinose concentrations as long as AraC is present.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1216
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1156
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870