Difference between revisions of "Part:BBa K510014:Design"
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Revision as of 19:47, 18 September 2011
pUC18R6KT-miniTn7BB-Km
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4799
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4799
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4805 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4799
Illegal BglII site found at 3212
Illegal BglII site found at 3483 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4799
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4799
Illegal XbaI site found at 4814
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518
Design Notes
Source
The pUC18R6KT-mini-Tn7-Km is a derivative of pUC18R6KT-mini-Tn7-Gm (BBa_K510012) by replacing the gentamycin resistance cassette by a kanamycin resistance cassette amplified from pSB4K5 with these primers: tatGCATGCCCATGGattggggctcactcaaagg and tatGCATGCCCATGGtcgacaatgtaactactag.