Difference between revisions of "Part:BBa K572005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone. | + | The gene had E,S and P sites. Thus, we used site directed mutagenesis and overlap PCR techniques to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone. |
===Source=== | ===Source=== | ||
Latest revision as of 21:28, 5 October 2011
Proteorhodopsin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene had E,S and P sites. Thus, we used site directed mutagenesis and overlap PCR techniques to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone.
Source
Source Organism : Marine gamma proteobacterium EBAC31A08.
We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene.
References
Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007). "Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host". PNAS 104 (13): 5590–5595. Bibcode 2007PNAS..104.5590M. doi:10.1073/pnas.0611470104. PMC 1838496. PMID 17372221.