Difference between revisions of "Part:BBa K537015:Design"
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+ | Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470 |
Latest revision as of 16:45, 18 September 2011
Promoter-Atrazine riboswitch-CheZ-mCherry-Double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 44 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1314
Illegal AgeI site found at 1426 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coming soon
Source
Atrazine Riboswitch + CheZ fusion - BBa_K537000
mCherry (mRFP1) C-Fusion - BBa_K537005
Strong promoter – Bba_J23119
Double terminator – Bba_B0015
References
Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470