Difference between revisions of "Part:BBa K528002"

 
 
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<partinfo>BBa_K528002 short</partinfo>
 
<partinfo>BBa_K528002 short</partinfo>
  
This device was used to determine BBa_B0032 strength relative to BBa_B0034.
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This device was used to determine as a standard measurement.
 +
 
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===Construct design===
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The device consists of BBa_K299504 expression vector J23100+B0034. E2050 mORANGE was used as reporter protein.
 +
 
 +
===Authors===
 +
Clonning:
 +
*Paweł Urbański
 +
*Elżbieta Jankowska
 +
*Krzysztof Szczepaniak
 +
*Dorota Kaja Sabat
 +
 +
The lab work was supervised by:
 +
*Michał Lower
 +
*Ania Olchowik
 +
 
 +
===Measurements===
 +
 
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All our mORANGE measurement results are summarized on the chart below:
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 +
https://static.igem.org/mediawiki/parts/7/79/Wyk5.png 
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For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
  
 
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Latest revision as of 15:08, 18 September 2011

RBS measurement device J23100 B0034 mORANGE

This device was used to determine as a standard measurement.

Construct design

The device consists of BBa_K299504 expression vector J23100+B0034. E2050 mORANGE was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Measurements

All our mORANGE measurement results are summarized on the chart below:

Wyk5.png

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 824
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]