Difference between revisions of "Part:BBa K528001"

Revision as of 14:37, 18 September 2011

RBS measurement device J23100 B0032 mORANGE

This device was used to determine BBa_B0032 strength relative to BBa_B0034.

The device consists of BBa_K299501 expression vector J23100+B0032. E2050 mORANGE was used as reporter protein.

Clonning:

The lab work was supervised by:

Using this construct we have determined that BBa_B0031 strength is 11,83% relative to BBa_B0034

All our mORANGE measurement results are summarized on the chart below:

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 825
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K528001 short</partinfo>
 This device was used to determine BBa_B0032 strength relative to BBa_B0034.
+===Construct design===
+The device consists of BBa_K299501 expression vector J23100+B0032. E2050 mORANGE was used as reporter protein.
+===Authors===
+Clonning:
+*Paweł Urbański
+*Elżbieta Jankowska
+*Krzysztof Szczepaniak
+*Dorota Kaja Sabat
+The lab work was supervised by:
+*Michał Lower
+*Ania Olchowik
+===Measurements===
+Using this construct we have determined that BBa_B0031 strength is 11,83% relative to BBa_B0034
+All our mORANGE measurement results are summarized on the chart below:
+https://static.igem.org/mediawiki/parts/7/79/Wyk5.png
+For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
 <!-- Add more about the biology of this part here