Difference between revisions of "Part:BBa K537006:Design"

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===Design Notes===
 
===Design Notes===
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Under construction  
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This BioBrick represents a C-terminal fusion part. The part contains a standard suffix and a “assembly standard 25” prefix (Freiburg Fusions/ RFC 25). This version of the Venus Fluorescent reporter (BBa_K354002) has the standard BioBrick suffix and prefix. The standard Biobrick prefix, however, is followed by another restriction site – specific for NgoMIV (which forms the Freiburg fusion prefix). The part lacks an ATG start codon (provided by N-terminal part). It can therefore be fused to any gene in a modular fashion via the modular construction strategy of protein fusion developed by the Freiburg 2007 team.
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===Source===
 
===Source===

Latest revision as of 13:52, 18 September 2011

Venus C-Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick represents a C-terminal fusion part. The part contains a standard suffix and a “assembly standard 25” prefix (Freiburg Fusions/ RFC 25). This version of the Venus Fluorescent reporter (BBa_K354002) has the standard BioBrick suffix and prefix. The standard Biobrick prefix, however, is followed by another restriction site – specific for NgoMIV (which forms the Freiburg fusion prefix). The part lacks an ATG start codon (provided by N-terminal part). It can therefore be fused to any gene in a modular fashion via the modular construction strategy of protein fusion developed by the Freiburg 2007 team.

Source

Venus fluorescent protein reporter derived from part BBa_K354002.

References