Difference between revisions of "Part:BBa K528000"
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===Measurements=== | ===Measurements=== | ||
Using this construct we have determined that BBa_B0031 strength is 69,73% relative to BBa_B0034 | Using this construct we have determined that BBa_B0031 strength is 69,73% relative to BBa_B0034 | ||
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Revision as of 14:07, 18 September 2011
RBS measurement device J23100 B0031 mORANGE
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
Construct design
The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Flow cytometer measurements done by Cherry Moreno
Measurements
Using this construct we have determined that BBa_B0031 strength is 69,73% relative to BBa_B0034
<img src="
" alt="ERROR" />
" alt="ERROR" />
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 826
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]