Difference between revisions of "Part:BBa P1010:Design"
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<partinfo>BBa_P1010 short</partinfo>
 
<partinfo>BBa_P1010 short</partinfo>
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===Design Notes===
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''ccdB''
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===Source===
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===References===
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CcdB, a natural analogue of the quinolone antibiotics (e.g., ciprofloxacin, enoxacin, etc.), binds to the DNA gyrase subunit A, the product of the gyrA gene, turning it into a cellular poison (Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944). E.coli strains that carry the gyrA462 mutation (e.g., DB3.1) are resistant to CcdB poisoning.

Revision as of 19:34, 8 June 2006

ccdB cell death gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114


ccdB

CcdB, a natural analogue of the quinolone antibiotics (e.g., ciprofloxacin, enoxacin, etc.), binds to the DNA gyrase subunit A, the product of the gyrA gene, turning it into a cellular poison (Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944). E.coli strains that carry the gyrA462 mutation (e.g., DB3.1) are resistant to CcdB poisoning.