Difference between revisions of "Part:BBa K528000"
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This device was used to determine BBa_B0031 strength relative to BBa_B0034.
 
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
  
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<b><font size="4">Construct design</font></b>
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===Construct design===
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<p align=justify>
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The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.  
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The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein. </p>
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===Authors===
 
===Authors===
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===Measurements===
 
===Measurements===
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Using this construct we have determined that {{{name}}} strength is '''{{{strength}}}''' relative to <partinfo>B0034</partinfo>
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Using this construct we have determined that BBa_B0031 strength is 69,73% relative to BBa_B0034
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:47, 18 September 2011

RBS measurement device J23100 B0031 mORANGE

This device was used to determine BBa_B0031 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Flow cytometer measurements done by Cherry Moreno

Measurements

Using this construct we have determined that BBa_B0031 strength is 69,73% relative to BBa_B0034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]