Difference between revisions of "Part:BBa K528000"
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The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein. </p> | The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein. </p> | ||
| − | + | ===Authors=== | |
| − | + | ||
| − | + | ||
| − | + | ||
Clonning: | Clonning: | ||
| − | * | + | *Paweł Urbański |
| − | * | + | *Elżbieta Jankowska |
| − | * | + | *Krzysztof Szczepaniak |
| + | *Dorota Kaja Sabat | ||
| + | |||
The lab work was supervised by: | The lab work was supervised by: | ||
| − | * | + | *Michał Lower |
*Ania Olchowik | *Ania Olchowik | ||
| − | |||
Flow cytometer measurements done by Cherry Moreno | Flow cytometer measurements done by Cherry Moreno | ||
Revision as of 13:27, 18 September 2011
RBS measurement device J23100 B0031 mORANGE
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
Construct design
The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Flow cytometer measurements done by Cherry Moreno
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 826
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]