Difference between revisions of "Part:BBa K592010:Experience"

 
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<partinfo>BBa_K592010</partinfo>
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<I>iGEM Groningen 2012</I>
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Our team has managed to couple this biobrick part with our promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for B. subtilis, engineered by team Groningen 2012), to allow color expression in B. subtilis. We utilized a strong RBS BBa_B0034 for pigment expression in E. coli and B. subtilis.
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The yellow colour was strongly visible in E.coli without any induction, while the expression in B. subtilis was more subtle (B. subtilis colony looks slightly yellow on the plate agar). However, under the induction of volatiles that were produced by the rotten meat, the expression of this part was strongly visible by human naked eyes.
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Revision as of 12:02, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K592010

User Reviews

UNIQa068ce40a5161470-partinfo-00000000-QINU UNIQa068ce40a5161470-partinfo-00000001-QINU

UNIQa068ce40a5161470-partinfo-00000002-QINU

BBa_K592010 iGEM Groningen 2012

Our team has managed to couple this biobrick part with our promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for B. subtilis, engineered by team Groningen 2012), to allow color expression in B. subtilis. We utilized a strong RBS BBa_B0034 for pigment expression in E. coli and B. subtilis.

The yellow colour was strongly visible in E.coli without any induction, while the expression in B. subtilis was more subtle (B. subtilis colony looks slightly yellow on the plate agar). However, under the induction of volatiles that were produced by the rotten meat, the expression of this part was strongly visible by human naked eyes.

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