Difference between revisions of "Part:BBa K592004"

Revision as of 18:47, 20 September 2011

YF1 blue-light sensor protein

YF1 is a fusion protein of YtvA (B subtilis) and FixL (B japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. In their article (see references) they showed that illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. They also showed that YF1 also controls gene expression in a light-dependent manner in vivo.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 724
Illegal AgeI site found at 258
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 157

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 __NOTOC__
 <partinfo>BBa_K592004 short</partinfo>
-YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by &#8764;1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
+YF1 is a fusion protein of YtvA (''B subtilis'') and FixL (''B japonicum''). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. In their article (see references) they showed that illumination of the fusion kinase YF1 reduced net kinase activity by &#8764;1000-fold in vitro. They also showed that YF1 also controls gene expression in a light-dependent manner in vivo.