Difference between revisions of "Part:BBa K590055"

Revision as of 19:23, 16 September 2011

RrD_C70V-PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.

Usage and Biology

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 470
Illegal NheI site found at 493
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1584
Illegal XhoI site found at 2366
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 126
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590055 short</partinfo>
-Plasmid that consists of Acyl-ACP Reductase rbs mutant Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
+This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], ribosomal binding site, [https://parts.igem.org/Part:BBa_K590038 mutant Aldehyde Decarbonylase] under the control of high consititutive promoter for alkane production.
 ===Usage and Biology===
+[[Image:Washington_Redesign_Data2.png]]
+When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
+[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
+[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]
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