Difference between revisions of "Part:BBa K546002"

Revision as of 18:57, 21 September 2011

Lux pL controlled luxR with lux pR controlled GFP(lva tag).

This device consists of two protein generators. The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein. The second generator ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored.

It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1828

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K546002 short</partinfo>
-This BioBrick system is able to notify the downstream activation of the lux R promoter of the 3OC6HSL Receiver Device.
+This device consists of two protein generators.
+The first generator  ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.
+The second generator  ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored.
+ It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.
 <!-- Add more about the biology of this part here