Difference between revisions of "Part:BBa K515100:Experience"
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<img src="https://static.igem.org/mediawiki/parts/5/58/ICL_Standard_curve_salkowski.png" width=500px/> | <img src="https://static.igem.org/mediawiki/parts/5/58/ICL_Standard_curve_salkowski.png" width=500px/> | ||
<img src="https://static.igem.org/mediawiki/parts/5/58/ICL_salkowski_cuvettes.JPG" width=400px/> | <img src="https://static.igem.org/mediawiki/parts/5/58/ICL_salkowski_cuvettes.JPG" width=400px/> | ||
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<p> Figure 1 - To make a calibration curve for the Salkowski assay, LB broth with different concentrations of synthetic auxin was added to salkowski reagent and OD measured at 530 nm. The picture on the right shows the visible colour change from yellow to red with increasing auxin concentration.</p> | <p> Figure 1 - To make a calibration curve for the Salkowski assay, LB broth with different concentrations of synthetic auxin was added to salkowski reagent and OD measured at 530 nm. The picture on the right shows the visible colour change from yellow to red with increasing auxin concentration.</p> | ||
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| − | <img src="https://static.igem.org/mediawiki/2011/b/bc/ICL_salkowskiAuxinGraph.png" width= | + | <img src="https://static.igem.org/mediawiki/2011/b/bc/ICL_salkowskiAuxinGraph.png" width="500px"/> |
<p><i>Results from salkowski assay with cell filtrate (add pore size)</i></p> | <p><i>Results from salkowski assay with cell filtrate (add pore size)</i></p> | ||
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===Applications of BBa_K515100=== | ===Applications of BBa_K515100=== | ||
Revision as of 12:58, 16 September 2011
Figure 1 - To make a calibration curve for the Salkowski assay, LB broth with different concentrations of synthetic auxin was added to salkowski reagent and OD measured at 530 nm. The picture on the right shows the visible colour change from yellow to red with increasing auxin concentration.
Results from salkowski assay with cell filtrate (add pore size)
Applications of BBa_K515100
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