Difference between revisions of "Part:BBa K537014"
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<partinfo>BBa_K537014 short</partinfo> | <partinfo>BBa_K537014 short</partinfo> | ||
| − | + | This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to the monomeric Red fluorescent reporter protein without a stop codon in between. The atrazine riboswitch-mRFP fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015). | |
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Latest revision as of 16:41, 18 September 2011
Promoter-Atrazine riboswitch-mCherry-Double terminator
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to the monomeric Red fluorescent reporter protein without a stop codon in between. The atrazine riboswitch-mRFP fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 52 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 683
Illegal AgeI site found at 795 - 1000COMPATIBLE WITH RFC[1000]