Difference between revisions of "Part:BBa K590002:Experience"

Revision as of 00:09, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K590002

User Reviews

The part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Background Magnetosome toolkit]. It served as the template to amplify the Gibson Cloning friendly version of J to make Part:BBa_K90017, and Part: BBa_K590020. The repetitive region in the middle of the mamJ sequence made it tricky to verify, we turned to the Komeili lab help, and we were suggested to use CGGTGTCCGTCTCCGA as the primer to verify the middle part of J sequence.

UNIQfca83bd518e73493-partinfo-00000000-QINU UNIQfca83bd518e73493-partinfo-00000001-QINU

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 ===User Reviews===
 The part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Background Magnetosome toolkit].
-It served as the template to amplify the Gibson Cloning friendly version of J to make [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590017 Part:BBa_K90017], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590020 Part: BBa_K590020]
+It served as the template to amplify the Gibson Cloning friendly version of J to make [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590017 Part:BBa_K90017], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590020 Part: BBa_K590020]. The repetitive region in the middle of the mamJ sequence made it tricky to verify, we turned to the Komeili lab help, and we were suggested to use CGGTGTCCGTCTCCGA as the primer to verify the middle part of J sequence.
 <!-- DON'T DELETE --><partinfo>BBa_K590002 StartReviews</partinfo>