Difference between revisions of "Part:BBa K590056"

 
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<partinfo>BBa_K590056 short</partinfo>
 
<partinfo>BBa_K590056 short</partinfo>
  
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Plasmid that consists of Acyl-ACP Reductase rbs mutant Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
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This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], ribosomal binding site, [https://parts.igem.org/Part:BBa_K590039 mutant Aldehyde Decarbonylase] under the control of high consititutive promoter for alkane production.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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[[Image:Washington_Redesign_Data2.png]]
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When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. 
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[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
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[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]
  
 
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Revision as of 19:39, 16 September 2011

RrD_C70K-PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.

Usage and Biology

Washington Redesign Data2.png

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.
GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1584
    Illegal XhoI site found at 2366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]