Difference between revisions of "Part:BBa K537003:Design"

Revision as of 17:16, 15 September 2011

Theophylline Riboswitch 1-Venus

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 686
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This biobrick part represents a composite of a theophylline riboswitch (type1/clone 8.1) upstream of a Venus fluorescent protein reporter (derived from part BBa_K354002).

Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.

This part represents a fused part, rather than a composite part that is made out of single biobricks.

Source

Topp and Gallivan JACS 2007 (and also BBa_K249026 and BBa_K411001) fusion with Venus reporter BBa_K354002.

References

Topp S, Gallivan JP. Guiding bacteria with small molecules and RNA. J Am Chem Soc 2007;129:6807-11

@@ Line 6: / Line 6: @@
 ===Design Notes===
-none
+This biobrick part represents a composite of a theophylline riboswitch (type1/clone 8.1) upstream of a Venus fluorescent protein reporter (derived from part BBa_K354002).
+Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.
+This part represents a fused part, rather than a composite part that is made out of single biobricks.
 ===Source===