Difference between revisions of "Part:BBa K523021"

 
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<partinfo>BBa_K523021 short</partinfo>
 
<partinfo>BBa_K523021 short</partinfo>
  
 
This is the pVIII localisation signal in BioSandwich format.
 
This is the pVIII localisation signal in BioSandwich format.
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BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 15:03, 15 September 2011

pVIII periplasmic localisation signal (for BioSandwich)

This is the pVIII localisation signal in BioSandwich format.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]