Difference between revisions of "Part:BBa K518004"

Latest revision as of 03:34, 27 September 2011

IPTG-inducible L-Asp producing device

AspA is an aspartate ammonia-lyase which catalyzes the equilibrium of aspartate and fumarate + ammonium ion. With enough amount of substrate, it produces aspartate. Aspartate can be used as a chemoattractant for Escherichia coli. In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 154
Illegal BglII site found at 1619
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1046
Illegal AgeI site found at 575
Illegal AgeI site found at 1427
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1086

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K518004 short</partinfo>
-AspA is an aspartate ammonia-lyase, which produces aspartate from fumarate and ammonium ion. Aspartate can be used as a chemoattractant for bacteria including Escherichia coli.
+AspA is an aspartate ammonia-lyase which catalyzes the equilibrium of aspartate and fumarate + ammonium ion. With enough amount of substrate, it produces aspartate. Aspartate can be used as a chemoattractant for Escherichia coli.
 In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.