Difference between revisions of "Part:BBa K518003"

Revision as of 14:56, 3 October 2011

LexA - a repressor for LexA-regulated SOS promoters -

Escherichia coli and other organisms have a gene cluster activated in case of emergency, which is called "SOS genes". It is well-documented that in E. coli, most of those SOS genes are repressed by LexA protein. When the cell is exposed to excess stress, RecA is up-produced, which promotes the self-degradation of LexA. As a result, many LexA-regulated genes become expressed. Combined with RecA and SOS promoters (for instance, sulAp), a stress-responsive gene-expression circuit was assembled. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 684

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 __NOTOC__
 <partinfo>BBa_K518003 short</partinfo>
 Escherichia coli and other organisms have a gene cluster activated in case of emergency, which is called "SOS genes". It is well-documented that in E. coli, most of those SOS genes are repressed by LexA protein. When the cell is exposed to excess stress, RecA is up-produced, which promotes the self-degradation of LexA. As a result, many LexA-regulated genes become expressed.
-Combined with RecA () and SOS promoters (for instance, ), a stress-responsive gene-expression circuit was assembled. See ().
+Combined with RecA and SOS promoters (for instance, [[Part:BBa_K518010 |sulAp]]), a stress-responsive gene-expression circuit was assembled. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].
 <!-- Add more about the biology of this part here